| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a; TIMM17A; MIMT17; TIM17; TIM17A; TIMM17 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human TMED5/p28 recombinant protein (Position: D33-K164). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TMED5/p28 Antibody Picoband® is an antibody for TMED5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TMED5 (translocase of inner mitochondrial membrane 17A); UniProt: Q9Y3A6
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 26 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-TMED5/p28 Antibody Picoband® catalog # A12319-1.
Biological background
Biological context: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Expression and localization notes: cellular localization: Mitochondrion inner membrane, Multi-pass membrane protein..
Common research applications
- Western blotting (WB): Compare TMED5 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TMED5 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify TMED5-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Transmembrane emp24 domain-containing protein 5 is a protein that in humans is encoded by the TMED5 gene. TMED5 is a 229 amino acid single-pass type I membrane protein that belongs to the EMP24/GP25L family and contains one GOLD domain. The gene that encodes TMED5 contains nearly 31,000 bases and maps to human chromosome 1p22.1. As the largest human chromosome, chromosome 1 spans about 260 million base pairs and makes up approximately 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion inner membrane, Multi-pass membrane protein.
- Research category: Cancer,Metabolism,Mitochondrial Metabolism,Organelle Proteins,Pathways and Processes,Protein Trafficking,Signal Transduction,Tumor Biomarkers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
