| Field | Specification |
|---|---|
| Target | |
| Alternative names | Anoctamin-1; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2; ANO1; DOG1; ORAOV2; TAOS2; TMEM16A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TMEM16A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17A32 is a rabbit monoclonal antibody that recognizes anoctamin-1 (gene ANO1) in human samples and is validated for IHC and flow cytometry. The predicted molecular weight is 114.1 kDa, and the supplier reports an observed band at about 114 kDa.
Anoctamin-1 (ANO1, also called TMEM16A) is a calcium-activated chloride channel. It drives chloride secretion in airway and gland epithelia, contributes to smooth muscle contraction, and is needed for pacemaker activity of the interstitial cells of Cajal in the gut, where it is also used as a marker.
| Target | Anoctamin-1 (gene ANO1; UniProt Q5XXA6, human) |
|---|---|
| Clone | 17A32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 114.1 kDa |
| Observed MW | 114 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|---|
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human TMEM16A.
Reactivity Notes
The supplier lists reactivity with human. UniProt places anoctamin-1 in the apical cell membrane and presynapse. Tissue expression noted by UniProt (human): Expressed in nasal epithelial cells (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
