| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stimulator of interferon genes protein;hSTING;Endoplasmic reticulum interferon stimulator;ERIS;Mediator of IRF3 activation;hMITA;Transmembrane protein 173;TMEM173;ERIS, MITA, STING; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM173, different from the related mouse sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects stimulator of interferon genes protein (gene STING1) in human and mouse samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 42.2 kDa, and the supplier reports an observed band at about 35 kDa.
STING (stimulator of interferon genes, STING1/TMEM173) is an endoplasmic reticulum adaptor in innate immunity. It is activated by cyclic dinucleotides, including cGAMP made by cGAS in response to cytosolic DNA, then moves to the Golgi and recruits TBK1 to activate IRF3 and NF-κB, driving type I interferon production. It is a major target in infection, autoinflammation and cancer immunology.
| Target | Stimulator of interferon genes protein (gene STING1; UniProt Q86WV6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.2 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, human Jurkat cells, mouse spleen tissue, mouse thymus tissue, mouse MH-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the C-terminal region of human TMEM173. It differs from the mouse sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places stimulator of interferon genes protein in the endoplasmic reticulum membrane, cytoplasm, Golgi apparatus membrane and endosome membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pengfei Wang et al. (2023) EFHD2 cooperates with E3 ubiquitin ligase Smurf1 to facilitate virus infection by promoting the degradation of TRAF6 in teleost fish. Journal of Virology. 10.1128/jvi.01176-23
Ming-Wei Fan et al. (2026) Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis. World Journal of Diabetes. 10.4239/wjd.v17.i2.114252
