| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transmembrane protein 65; TMEM65 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TMEM65, identical to the related mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets transmembrane protein 65 (gene TMEM65) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 25.5 kDa, and the supplier reports an observed band at about 20 kDa.
TMEM65 is a mitochondrial inner membrane protein that supports sodium-dependent calcium efflux from mitochondria. It associates with the Na+/Ca2+ exchanger NCLX (SLC8B1) and appears to promote its activity, although its exact mechanism is still unclear. TMEM65 is studied in mitochondrial calcium handling and in heart and muscle physiology.
| Target | Transmembrane protein 65 (gene TMEM65; UniProt Q6PI78, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.5 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human 293T cells, human MCF-7 cells, rat brain tissue, rat C6 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SiHa cells, ANA-1 cells, RH35 cells.
Immunogen
Synthetic peptide from the middle region of human TMEM65. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transmembrane protein 65 in the mitochondrion inner membrane and cell membrane. Tissue expression noted by UniProt (human): Predominantly expressed the ventricular tissue (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
