| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transmembrane protease serine 2; Serine protease 10; TMPRSS2; PRSS10 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TMPRSS2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23T34 is a rabbit monoclonal antibody that recognizes transmembrane protease serine 2 (gene TMPRSS2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 53.9 kDa, and the supplier reports an observed band at about 54 kDa.
TMPRSS2 is an androgen-regulated type II transmembrane serine protease highly expressed in prostate and airway epithelium. It activates substrates such as pro-HGF and cleaves the spike proteins of influenza viruses and coronaviruses, including SARS-CoV-2, enabling viral entry. TMPRSS2–ERG gene fusions are common in prostate cancer, making it a focus of oncology and virology research.
| Target | Transmembrane protease serine 2 (gene TMPRSS2; UniProt O15393, human) |
|---|---|
| Clone | 23T34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.9 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:60 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Synthetic peptide based on the sequence of human TMPRSS2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transmembrane protease serine 2 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in several tissues that comprise large populations of epithelial cells with the highest level of transcripts measured in the prostate gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
