| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptosis-associated speck-like protein containing a CARD; mASC; PYD and CARD domain-containing protein; Pycard; Asc |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse TMS1/ASC/PYCARD recombinant protein (Position: R3-S193). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes apoptosis-associated speck-like protein containing a CARD (gene Pycard) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 21.5 kDa, and the supplier reports an observed band at about 22–25 kDa.
ASC (PYCARD) is an adaptor protein with a pyrin domain and a CARD. When sensors such as NLRP3, AIM2 or NLRC4 are triggered, ASC polymerizes into a large "speck" that recruits and activates caspase-1, leading to IL-1β and IL-18 maturation and pyroptosis, so it is a standard readout of inflammasome activation.
| Target | Apoptosis-associated speck-like protein containing a CARD (gene Pycard; UniProt Q9EPB4, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 21.5 kDa |
| Observed MW | 22–25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, rat spleen tissue, rat thymus tissue, rat heart tissue, mouse lung tissue, mouse spleen tissue, mouse thymus tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Recombinant mouse TMS1/ASC/PYCARD fragment (Arg3–Ser193), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places apoptosis-associated speck-like protein containing a CARD in the cytoplasm, inflammasome, endoplasmic reticulum and mitochondrion. Tissue expression noted by UniProt (mouse): Expressed in small intestine, colon, thymus, spleen, brain, heart, skeletal muscle, kidney, lung and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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