| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tumor necrosis factor (gene Tnf) in rat samples and is validated for Western blot. The predicted molecular weight is 25.8 kDa, and the supplier reports an observed band at about 26 kDa.
Tumor necrosis factor (TNF, TNF-α) is a pro-inflammatory cytokine produced mainly by activated macrophages and T cells. Acting through the receptors TNFR1 and TNFR2, it activates NF-κB and MAP kinase pathways and can trigger apoptosis or necroptosis, driving fever and inflammation. TNF is a central target in rheumatoid arthritis and inflammatory bowel disease research and a standard readout in immunology studies.
| Target | Tumor necrosis factor (gene Tnf; UniProt P16599, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.8 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat) |
|---|
Samples with a confirmed band (WB): PC-12 cells, rat spleen tissue, rat brain tissue, rat kidney tissue, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant rat TNF alpha fragment (Asp89–Leu235), expressed in E. coli. Sequence identity with the mouse ortholog: 95%.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places tumor necrosis factor in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Hao-Nan et al. (2021) Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3. Journal of Neuroinflammation. 10.1186/s12974-021-02198-9
Miao-Miao Zhang et al. (2025) Red nucleus IL-15 facilitates the development of neuropathic pain in male rats by inducing inflammatory factors: implying the involvement of NF-κB and p38 MAPK. Brain Behavior and Immunity. 10.1016/j.bbi.2025.106137
Peng Li et al. (2020) DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke. Journal of Neuroinflammation. 10.1186/s12974-020-01764-x
Nan Li et al. (2024) Anthrahydroquinone‑2,6‑disulfonate attenuates PQ‑induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of the PI3K/AKT/eNOS pathway. International Journal of Molecular Medicine. 10.3892/ijmm.2024.5387
Wu Baoguo et al. (2022) LncRNA-UC.25 + shRNA Alleviates P2Y14 Receptor–Mediated Diabetic Neuropathic Pain via STAT1. Molecular Neurobiology. 10.1007/s12035-022-02925-0
Liejun Liao et al. (2021) Bioinformatic and biochemical studies of formononetin against liver injure. Life Sciences. 10.1016/j.lfs.2021.119229
Jingxuan Yu et al. (2025) Propionic acid mediates the renoprotective effects of fecal microbiota transplantation against ischemia–reperfusion injury via upregulating GPR43. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2025.1616164
Hua Liu et al. (2024) Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118469
Yang Ping et al. (2020) A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2020.109826
Du Junpei et al. (2025) Resident Macrophages in the Cervical Sympathetic Ganglia Participate in P2Y12 Receptor Mediated Diabetic Cardiac Autonomic Neuropathy. Molecular Neurobiology. 10.1007/s12035-025-04883-9
