| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lymphotoxin-alpha;LT-alpha;TNF-beta;Tumor necrosis factor ligand superfamily member 1;LTA;TNFB, TNFSF1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TNF beta, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes lymphotoxin-alpha (gene LTA) in human samples and is validated for Western blot. The predicted molecular weight is 22.3 kDa, and the supplier reports an observed band at about 6 kDa.
Lymphotoxin-alpha (LTA, also called TNF-β) is a TNF-family cytokine made mainly by activated lymphocytes. As a soluble homotrimer it signals through TNFR1, TNFR2 and HVEM, and as a membrane complex with lymphotoxin-beta it engages the lymphotoxin-beta receptor, which is essential for lymph node and other lymphoid organ development; it is studied in inflammation and lymphoid tissue organization.
| Target | Lymphotoxin-alpha (gene LTA; UniProt P01374, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.3 kDa |
| Observed MW | 6 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): recombinant human TNF beta protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the middle region of human TNF beta. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes lymphotoxin-alpha as secreted and also located in the membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
