| Field | Specification |
|---|---|
| Target | |
| Alternative names | Target of EGR1 protein 1; TOE1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TOE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27T99 is a rabbit monoclonal antibody that recognizes target of EGR1 protein 1 (gene TOE1) in human samples and is validated for Western blot. The predicted molecular weight is 56.5 kDa, and the supplier reports an observed band at about 57 kDa.
Target of EGR1 protein 1 (TOE1) is a nuclear deadenylase first identified as a gene induced by the transcription factor EGR1. It helps process the 3′ ends of small nuclear RNAs (snRNAs) during their maturation and can slow cell growth, partly by inducing the CDK inhibitor p21. TOE1 mutations cause pontocerebellar hypoplasia type 7.
| Target | Target of EGR1 protein 1 (gene TOE1; UniProt Q96GM8, human) |
|---|---|
| Clone | 27T99 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.5 kDa |
| Observed MW | 57 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TOE1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places target of EGR1 protein 1 in the nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
