| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 4;hToll;CD284;TLR4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes toll-like receptor 4 (gene TLR4) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 95.7 kDa, and the supplier reports an observed band at about 80 kDa.
Toll-like receptor 4 (TLR4) is the receptor for bacterial lipopolysaccharide (LPS), working with the co-receptor MD-2 (LY96) and CD14. It signals from the cell surface through MyD88 and, after internalization, through TRIF, activating NF-κB, IRF3 and inflammatory cytokine production. TLR4 is central to research on sepsis, endotoxin responses and sterile inflammation.
| Target | Toll-like receptor 4 (gene TLR4; UniProt O00206, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 95.7 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells, SMMC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human TLR4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places toll-like receptor 4 in the cell membrane, early endosome and cell projection. Tissue expression noted by UniProt (human): Highly expressed in placenta, spleen and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Daiyang Xia et al. (2021) Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner. Journal of Hazardous Materials. 10.1016/j.jhazmat.2021.125239
Wang Wence et al. (2019) Ochratoxin A induces liver inflammation: involvement of intestinal microbiota. Microbiome. 10.1186/s40168-019-0761-z
Hongli Li et al. (2017) Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells. Theranostics. 10.7150/thno.17584
Xuehai Jia et al. (2022) Total glycosides of Rhodiola rosea L. attenuate LPS-induced acute lung injury by inhibiting TLR4/NF-κB pathway. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2022.114186
Han Zhang et al. (2021) HMGB1 is a key factor for tamoxifen resistance and has the potential to predict the efficacy of CDK4/6 inhibitors in breast cancer. Cancer Science. 10.1111/cas.14813
Haoran Liu et al. (2019) H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway. EBioMedicine. 10.1016/j.ebiom.2019.10.059
Wang Liyuan et al. (2017) Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens. Frontiers in Immunology. 10.3389/fimmu.2017.01856
Wang Wei et al. (2021) Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation. ACTA Pharmacologica Sinica. 10.1038/s41401-021-00714-4
Ke Yang et al. (2020) Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2020.01.026
Sun Lin et al. (2017) Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner. Journal of Neuroinflammation. 10.1186/s12974-017-1008-1
