| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial import receptor subunit TOM20 homolog;Mitochondrial 20 kDa outer membrane protein;Outer mitochondrial membrane receptor Tom20;TOMM20;KIAA0016; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TOMM20 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOC-20 is a rabbit monoclonal antibody that recognizes mitochondrial import receptor subunit TOM20 homolog (gene TOMM20) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 16.3 kDa, and the supplier reports an observed band at about 16 kDa.
TOM20 (TOMM20) is a receptor subunit of the translocase of the outer mitochondrial membrane (TOM complex). Together with TOM22, it recognizes the presequences of nuclear-encoded mitochondrial proteins made in the cytosol and hands them to the TOM40 channel for import. TOMM20 is widely used as a marker of mitochondria in imaging and Western blot studies.
| Target | Mitochondrial import receptor subunit TOM20 homolog (gene TOMM20; UniProt Q15388, human) |
|---|---|
| Clone | EOC-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.3 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human 293T cells, human K562 cells, human HeLa cells, rat kidney tissue, rat stomach tissue, mouse kidney tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human TOMM20.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial import receptor subunit TOM20 homolog in the mitochondrion outer membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xie Xiao-Qing et al. (2023) Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria. Cell Research. 10.1038/s41422-022-00766-z
Yu Meng et al. (2025) HCMV immediate-early protein IE2 induces neurotoxicity and hearing loss by disrupting TSC2-mTOR signaling and metabolic homeostasis. Journal of Neuroinflammation. 10.1186/s12974-025-03646-6
Chenglong Li et al. (2025) Inhibition of CPT1A activates the cGAS/STING pathway to enhance neutrophil-mediated tumor abrogation in triple-negative breast cancer. Cancer Letters. 10.1016/j.canlet.2025.217991
Peng Wang et al. (2023) Astaxanthin Protects against Alcoholic Liver Injury via Regulating Mitochondrial Redox Balance and Calcium Homeostasis. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.3c05529
Gewenhan Chen et al. (2025) Wnt/β-catenin/ATF3 signaling promotes the progression from acute kidney injury to chronic kidney disease by regulating mitophagy in mice. Biochemical Pharmacology. 10.1016/j.bcp.2025.117444
Li Huijun et al. (2022) Astragaloside IV alleviates senescence of vascular smooth muscle cells through activating Parkin-mediated mitophagy. Human Cell. 10.1007/s13577-022-00758-6
Fan Zhang et al. (2024) Ruthenium Red Alleviates Post-Resuscitation Myocardial Dysfunction by Upregulating Mitophagy through Inhibition of USP33 in a Cardiac Arrest Rat Model. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176633
Wen-xiu Sun et al. (2026) Oxidative Stress and Mitophagy in Rats With Nitroglycerin/Inflammatory Soup−Induced Migraine: A Comparative Study. Pain Research & Management. 10.1155/prm/5584799
Zhaokai Li et al. (2026) PROTAC-Mediated Degradation of Class I HDACs by JPS016 Alleviates Septic Cardiomyopathy via Mitophagy-Driven Exopher Formation and Mitochondrial Quality Control. International Journal of Biochemistry & Cell Biology. 10.1016/j.biocel.2026.106910
