| Field | Specification |
|---|---|
| Target | |
| Alternative names | Torsin-1A-interacting protein 1; Lamin-associated protein 1B; LAP1B; TOR1AIP1; LAP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TOR1AIP1 recombinant protein (Position: M1-D445). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects torsin-1A-interacting protein 1 (gene TOR1AIP1) in human samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 66.2 kDa, and the supplier reports an observed band at about 66 kDa.
Lamina-associated polypeptide 1 (LAP1, TOR1AIP1) is an inner nuclear membrane protein that binds A- and B-type lamins. It activates the ATPase activity of torsin-1A and torsin-1B and keeps them at the nuclear envelope, contributing to nuclear membrane integrity and lamina organization. TOR1AIP1 mutations are linked to muscular dystrophy and cardiomyopathy.
| Target | Torsin-1A-interacting protein 1 (gene TOR1AIP1; UniProt Q5JTV8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 66.2 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): SiHa cells.
Immunogen
Recombinant human TOR1AIP1 fragment (Met1–Asp445), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places torsin-1A-interacting protein 1 in the nucleus inner membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in muscle, liver and kidney... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
