| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TP53 recombinant protein (Position: M1-D186). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cellular tumor antigen p53 (gene TP53) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA.
p53 (TP53) is a tumor suppressor transcription factor that responds to DNA damage and other stresses by activating genes for cell cycle arrest, DNA repair, senescence or apoptosis. It is mutated in about half of human cancers, making it one of the most studied proteins in cancer biology, with rat models widely used alongside human cells.
| Target | Cellular tumor antigen p53 (gene TP53; UniProt P04637, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL/ml (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL/ml (Human) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human TP53 fragment (Met1–Asp186), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cellular tumor antigen p53 in the cytoplasm, nucleus, endoplasmic reticulum and mitochondrion matrix. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
