| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tryptophan 5-hydroxylase 1;1.14.16.4;Tryptophan 5-monooxygenase 1;TPH1;TPH, TPRH, TRPH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human TPH1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TPH1/Tph Rabbit Monoclonal Antibody is an antibody targeting TPH1. Common applications include WB, IHC, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EDH-20; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 43 kDa; calculated MW: 50985 MW.
Boster Bio Anti-TPH1/Tph Rabbit Monoclonal Antibody catalog # M01626. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TPH1 — Tryptophan 5-hydroxylase 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EDH-20; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 43 kDa; Calculated: 50985 MW
Biological background
Protein function (datasheet): t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. .
Cellular localization (datasheet): Cytoplasm, perinuclear region . Cell membrane ; Lipid-anchor. Cell junction, synapse, synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). .
Tissue details (datasheet): Isoform 2 seems to be less widely expressed than isoform 1.
Research relevance and current trends
- Commonly studied in contexts related to Amino Acid Metabolism,Amino Acids,Cancer,Cancer Metabolism,Endocrine Metabolism,Energy Metabolism,Energy Transfer Pathways,Hormone Biosynthesis,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Neuroscience,Neurotransmitter,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
