| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tryptophan 5-hydroxylase 1;1.14.16.4;Tryptophan 5-monooxygenase 1;TPH1;TPH, TPRH, TRPH; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TPH1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EDH-20 is a rabbit monoclonal antibody that recognizes tryptophan 5-hydroxylase 1 (gene TPH1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 51.0 kDa, and the supplier reports an observed band at about 70 kDa.
Tryptophan hydroxylase 1 (TPH1) catalyzes the first and rate-limiting step of serotonin synthesis, converting L-tryptophan into 5-hydroxytryptophan. It is the isoform found mainly in peripheral tissues such as gut enterochromaffin cells and the pineal gland, so it is studied in gut serotonin signaling, platelet function and melatonin production.
| Target | Tryptophan 5-hydroxylase 1 (gene TPH1; UniProt P17752, human) |
|---|---|
| Clone | EDH-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.0 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TPH1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Seems to be less widely expressed than isoform 1. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Feng Hong et al. (2026) A Balloon Electrochemical Sensor for Conformal Interfacing With Intestinal Wall and Real-Time Monitoring of Serotonin Release. Small. 10.1002/smll.202506988
Qiaoxia Wang et al. (2024) Exploring the mechanism and effective compounds of Changan Granule on diarrhea-predominant irritable bowel syndrome via regulating 5-hydroxytryptamine signaling pathway in brain-gut axis. Phytomedicine. 10.1016/j.phymed.2024.156350
