| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tryptophan 5-hydroxylase 2; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2; TPH2; NTPH |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant protein with human THP2 (Position: M1-I490). |
| Molecular weight | |
| Purification | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 31T01 is a rabbit monoclonal antibody that recognizes tryptophan 5-hydroxylase 2 (gene TPH2) in mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 56.1 kDa, and the supplier reports an observed band at about 56 kDa.
Tryptophan hydroxylase 2 (TPH2) is the neuronal isoform of the enzyme that performs the rate-limiting step of serotonin synthesis, using iron, oxygen and tetrahydrobiopterin to convert L-tryptophan into 5-hydroxytryptophan. It is expressed in serotonergic neurons of the raphe nuclei and is studied in mood, anxiety and sleep research.
| Target | Tryptophan 5-hydroxylase 2 (gene TPH2; UniProt Q8IWU9, human) |
|---|---|
| Clone | 31T01 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.1 kDa |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:3000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant protein with human THP2 (Position: M1-I490).
Reactivity Notes
The supplier lists reactivity with mouse and rat. Tissue expression noted by UniProt (human): Brain specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
