| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thiopurine S-methyltransferase; Thiopurine methyltransferase; TPMT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TPMT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29T23 is a rabbit monoclonal antibody that recognizes thiopurine S-methyltransferase (gene TPMT) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 28.2 kDa, and the supplier reports an observed band at about 30 kDa.
Thiopurine S-methyltransferase (TPMT) methylates thiopurine drugs such as 6-mercaptopurine and 6-thioguanine using S-adenosylmethionine, which lowers the amount converted into active thioguanine nucleotides. Inherited low TPMT activity increases the risk of severe toxicity from these drugs, making TPMT a classic example in pharmacogenetics.
| Target | Thiopurine S-methyltransferase (gene TPMT; UniProt P51580, human) |
|---|---|
| Clone | 29T23 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.2 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TPMT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places thiopurine S-methyltransferase in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
