| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tubulin polymerization-promoting protein; TPPP; 25 kDa brain-specific protein; TPPP/p25; p24; p25-alpha; TPPP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TPPP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21T61 is a rabbit monoclonal antibody that recognizes tubulin polymerization-promoting protein (gene TPPP) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 24 kDa.
Tubulin polymerization-promoting protein (TPPP, also called p25α) is a regulator of microtubule assembly expressed mainly in oligodendrocytes. It promotes microtubule nucleation at Golgi outposts and is needed for elongation of myelin sheaths. TPPP also accumulates in α-synuclein inclusions and is studied in multiple system atrophy and Parkinson disease.
| Target | Tubulin polymerization-promoting protein (gene TPPP; UniProt O94811, human) |
|---|---|
| Clone | 21T61 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.7 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TPPP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tubulin polymerization-promoting protein in the Golgi outpost, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
