| Field | Specification |
|---|---|
| Target | |
| Alternative names | Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TPX2 recombinant protein (Position: D15-Q93). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects targeting protein for Xklp2 (gene TPX2) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 85.7 kDa, and the supplier reports an observed band at about 86 kDa.
TPX2 is a microtubule-associated protein required for building the mitotic spindle. Released near chromosomes by Ran-GTP, it promotes microtubule nucleation and binds and activates the kinase Aurora A, targeting it to spindle microtubules. TPX2 is frequently overexpressed in tumors and is studied in cell division and cancer biology.
| Target | Targeting protein for Xklp2 (gene TPX2; UniProt Q9ULW0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 85.7 kDa |
| Observed MW | 86 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human U937 cells, human A431 cells, human Caco-2 cells, human K562 cells, human HepG2 cells, human Daudi cells, rat testis tissue, mouse stomach tissue, mouse testis tissue, mouse pancrease tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TPX2 fragment (Asp15–Gln93), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places targeting protein for Xklp2 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in lung carcinoma cell lines but not in normal lung tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhong Xiaodan et al. (2021) HMGB3 is Associated With an Unfavorable Prognosis of Neuroblastoma and Promotes Tumor Progression by Mediating TPX2. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2021.769547
Long Niya et al. (2020) CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells. Cancer Cell International. 10.1186/s12935-020-01454-x
