| Field | Specification |
|---|---|
| Target | |
| Alternative names | TNF receptor-associated factor 2;6.3.2.-;E3 ubiquitin-protein ligase TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3;TRAF2;TRAP3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRAF2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HOG-20 is a rabbit monoclonal antibody that recognizes TNF receptor-associated factor 2 (gene TRAF2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 55.9 kDa, and the supplier reports an observed band at about 50 kDa.
TRAF2 is a RING-domain E3 ubiquitin ligase and adaptor that is recruited to TNF family receptors such as TNFR1 and TNFR2. By building Lys-63-linked ubiquitin chains on partners including RIPK1 and cIAPs, it helps switch on NF-κB and JNK signaling and balances cell survival against apoptosis, so it is central to TNF signaling research.
| Target | TNF receptor-associated factor 2 (gene TRAF2; UniProt Q12933, human) |
|---|---|
| Clone | HOG-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.9 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:40 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human Caco-2 cells, human Jurkat cells, rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TRAF2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TNF receptor-associated factor 2 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zheng Yujia et al. (2025) Characterization of tertiary lymphoid structure identifies competitive binding of CD40 and STING with TRAF2 driving IRF4-mediated B cell activation in esophageal squamous cell carcinoma. Cancer Gene Therapy. 10.1038/s41417-025-00944-2
Bin Liu et al. (2025) IRE1α/TRAF2/NF-κB pathway promotes apoptosis via regulating inflammatory cytokines and aggravates brain injury after SAH. Journal of Stroke & Cerebrovascular Diseases. 10.1016/j.jstrokecerebrovasdis.2025.108288
