| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | TNF receptor-associated factor 4;Cysteine-rich domain associated with RING and Traf domains protein 1;Metastatic lymph node gene 62 protein;MLN 62;RING finger protein 83;TRAF4;CART1, MLN62, RNF83; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human TRAF4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TRAF4 Rabbit Monoclonal Antibody is an antibody targeting TRAF4. Common applications include WB, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: BGH-20; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 68 kDa; calculated MW: 53543 MW.
Boster Bio Anti-TRAF4 Rabbit Monoclonal Antibody catalog # M03069. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: TRAF4 — TNF receptor-associated factor 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: BGH-20; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 68 kDa; Calculated: 53543 MW
Biological background
Protein function (datasheet): Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. .
Cellular localization (datasheet): Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Cell junction, tight junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton .
Tissue details (datasheet): Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Cell Death,Cytoplasmic,Growth Factors/Hormones,Intracellular,Invasion/Microenvironment,Receptors,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
