| Field | Specification |
|---|---|
| Target | |
| Alternative names | TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; RING-type E3 ubiquitin transferase TRAF6; Traf6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse TRAF6 recombinant protein (Position: Q14-D512). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets TNF receptor-associated factor 6 (gene Traf6) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 60.1 kDa, and the supplier reports an observed band at about 60–65 kDa.
TRAF6 is an E3 ubiquitin ligase that builds Lys63-linked ubiquitin chains downstream of IL-1 receptors, Toll-like receptors, RANK and the TNF receptor superfamily. These chains activate the TAK1 kinase complex and in turn NF-κB and MAP kinase signaling, and TRAF6 is also essential for osteoclast differentiation.
| Target | TNF receptor-associated factor 6 (gene Traf6; UniProt P70196, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 60.1 kDa |
| Observed MW | 60–65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat RH35 cells, mouse thymus tissue, mouse spleen tissue, mouse RAW2467 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HEPA 1–6 cells.
Immunogen
Recombinant mouse TRAF6 fragment (Gln14–Asp512), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places TNF receptor-associated factor 6 in the cytoplasm and nucleus. Tissue expression noted by UniProt (mouse): Highly expressed in brain, lung, liver, skeletal muscle, and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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