| Field | Specification |
|---|---|
| Target | |
| Alternative names | TNF receptor-associated factor 6;6.3.2.-;E3 ubiquitin-protein ligase TRAF6;Interleukin-1 signal transducer;RING finger protein 85;TRAF6;RNF85; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRAF6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BOF-20 is a rabbit monoclonal antibody that recognizes TNF receptor-associated factor 6 (gene TRAF6) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 59.6 kDa, and the supplier reports an observed band at about 58 kDa.
TRAF6 is an E3 ubiquitin ligase that builds Lys63-linked ubiquitin chains downstream of IL-1 receptors, Toll-like receptors, RANK and the TNF receptor superfamily. These chains activate the TAK1 kinase complex and in turn NF-κB and MAP kinase signaling, and TRAF6 is also essential for osteoclast differentiation.
| Target | TNF receptor-associated factor 6 (gene TRAF6; UniProt Q9Y4K3, human) |
|---|---|
| Clone | BOF-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.6 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50–1:200 |
Samples with confirmed staining (IHC): rat ovary tissue, rat stomach tissue, human prostate cancer tissue, human astrocytoma tissue, mouse skeletal muscle - gastrocnemius tissue, human colon carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human TRAF6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TNF receptor-associated factor 6 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
