| Field | Specification |
|---|---|
| Target | |
| Alternative names | TNF receptor-associated factor 6;6.3.2.-;E3 ubiquitin-protein ligase TRAF6;Interleukin-1 signal transducer;RING finger protein 85;TRAF6;RNF85; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRAF6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BOG-20 is a rabbit monoclonal antibody that recognizes TNF receptor-associated factor 6 (gene TRAF6) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 59.6 kDa, and the supplier reports an observed band at about 58 kDa.
TRAF6 is an E3 ubiquitin ligase that builds Lys63-linked ubiquitin chains downstream of IL-1 receptors, Toll-like receptors, RANK and the TNF receptor superfamily. These chains activate the TAK1 kinase complex and in turn NF-κB and MAP kinase signaling, and TRAF6 is also essential for osteoclast differentiation.
| Target | TNF receptor-associated factor 6 (gene TRAF6; UniProt Q9Y4K3, human) |
|---|---|
| Clone | BOG-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.6 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human TRAF6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TNF receptor-associated factor 6 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ye Jin et al. (2023) Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis. Environmental Pollution. 10.1016/j.envpol.2023.122407
Qi Zhang et al. (2022) Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway. Nutrients. 10.3390/nu14193978
Chen Yang et al. (2023) The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish. Communications Biology. 10.1038/s42003-023-04711-7
Ya Chen et al. (2022) eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish. Journal of Biological Chemistry. 10.1016/j.jbc.2022.101730
Pengfei Wang et al. (2023) EFHD2 cooperates with E3 ubiquitin ligase Smurf1 to facilitate virus infection by promoting the degradation of TRAF6 in teleost fish. Journal of Virology. 10.1128/jvi.01176-23
Shen Yi et al. (2022) Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro. Chinese Medicine. 10.1186/s13020-022-00627-2
Shen Yi et al. (2024) Morinda officinalis iridoid glycosides, as an inhibitor of GSK-3β, alleviates rheumatoid arthritis through inhibition of NF-κB and JAK2/STAT3 pathway. Frontiers in Pharmacology. 10.3389/fphar.2024.1435274
Chunlian Tian et al. (2021) The protective effect of rutin against lipopolysaccharide induced acute lung injury in mice based on the pharmacokinetic and pharmacodynamic combination model. Journal of Pharmaceutical and Biomedical Analysis. 10.1016/j.jpba.2021.114480
Wei Wang et al. (2024) Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12. Toxicology and Applied Pharmacology. 10.1016/j.taap.2024.116916
Wu Liyan et al. (2018) IL-33 Can Promote the Process of Pulmonary Fibrosis by Inducing the Imbalance Between MMP-9 and TIMP-1. Inflammation. 10.1007/s10753-018-0742-6
