| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tax1-binding protein 1; TRAF6-binding protein; TAX1BP1; T6BP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRAF6BP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23T27 is a rabbit monoclonal antibody that recognizes tax1-binding protein 1 (gene TAX1BP1) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 90.9 kDa, and the supplier reports an observed band at about 91 kDa.
TAX1BP1 is a ubiquitin-binding adaptor that works with the deubiquitinase A20 (TNFAIP3) to switch off NF-κB and IRF3 signaling after inflammatory or antiviral stimulation. It also acts as a selective autophagy receptor that helps clear protein aggregates and intracellular bacteria, and it was first identified as a binding partner of the HTLV-1 Tax protein.
| Target | Tax1-binding protein 1 (gene TAX1BP1; UniProt Q86VP1, human) |
|---|---|
| Clone | 23T27 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 90.9 kDa |
| Observed MW | 91 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human RT4 cells, human SIHA cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TRAF6BP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tax1-binding protein 1 in the cytoplasm, mitochondrion, preautophagosomal structure and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Expressed in all tissues tested. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
