| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor ligand superfamily member 10;TNF-related apoptosis-inducing ligand;Protein TRAIL;CD253;Tnfsf10;Trail; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse TRAIL recombinant protein (Position: Y39-N291). Mouse TRAIL shares 66.3% amino acid (aa) sequence identity with human TRAIL. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tumor necrosis factor ligand superfamily member 10 (gene Tnfsf10) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 33.5 kDa, and the supplier reports an observed band at about 20 kDa.
TRAIL (TNFSF10, Apo2L) is a TNF family cytokine that induces apoptosis by engaging the death receptors DR4 (TNFRSF10A) and DR5 (TNFRSF10B). Its activity is tuned by decoy receptors, including TNFRSF10C, TNFRSF10D and osteoprotegerin, which bind TRAIL without triggering cell death. TRAIL is studied in cancer biology, immune surveillance and apoptosis research.
| Target | Tumor necrosis factor ligand superfamily member 10 (gene Tnfsf10; UniProt P50592, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 33.5 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HEPA1–6 cells, NIH3T3 cells, PC12 cells, NRK cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse TRAIL fragment (Tyr39–Asn291), expressed in E. coli. Sequence identity with the human ortholog: 66.3%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes tumor necrosis factor ligand superfamily member 10 as secreted and also located in the cell membrane. Tissue expression noted by UniProt (mouse): Widespread. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
