| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serotransferrin;Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF;PRO1400; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Transferrin, different from the related mouse and rat sequences by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serotransferrin (gene TF) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 77.1 kDa, and the supplier reports an observed band at about 77 kDa.
Serotransferrin (TF) is the main iron-transport protein of blood plasma, made mostly by the liver. Each molecule binds two ferric iron ions and delivers them to cells through the transferrin receptor, and transferrin levels and saturation are standard measures in iron metabolism research.
| Target | Serotransferrin (gene TF; UniProt P02787, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 77.1 kDa |
| Observed MW | 77 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human placenta tissue, human hepatocellular carcinoma tumor tissue, human hepatocellular carcinoma paracancerous tissue, human HepG2 cells, rat liver tissue, mouse liver tissue, mouse Hepa1/6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to PVDF membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the N-terminal region of human transferrin. It differs from the mouse and rat sequences at five residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes serotransferrin as a secreted protein. Tissue expression noted by UniProt (human): Expressed by the liver and secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiang Yufang et al. (2021) Comparison of the sensitivity of Western blotting between PVDF and NC membranes. Scientific Reports. 10.1038/s41598-021-91521-8
Sun Xi et al. (2020) Nephrotoxicity Profile of Cadmium Revealed by Proteomics in Mouse Kidney. Biological Trace Element Research. 10.1007/s12011-020-02312-7
Y-L Sun et al. (2011) Comparative Proteomic Analysis of Follicular Fluids from Normal and Cystic Follicles in Sows. Reproduction in Domestic Animals. 10.1111/j.1439-0531.2011.01760.x
Zhu Wei et al. (2011) Protective effect and mechanism of sodium tanshinone II A sulfonate on microcirculatory disturbance of small intestine in rats with sepsis. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-011-0470-8
