| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein disulfide-isomerase A2;5.3.4.1;Pancreas-specific protein disulfide isomerase;PDIp;PDIA2;PDIP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ACP5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEGH-1; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-TRAP ACP5 Monoclonal Antibody catalog # M03277-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ACP5 (Protein disulfide-isomerase A2).
- Antibody format: Monoclonal; clone AEGH-1; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ACP5 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. . Reported cellular localization context: Endoplasmic reticulum lumen . Tissue expression notes (as provided): Highly expressed in pancreas (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: Cofactors, Vitamins/Minerals,Cytoskeleton/ECM,Extracellular Matrix,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Organelle Proteins,Pathways and Processes,Protein Trafficking,Signal Transduction,Structures,Vitamins/Minerals.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate ACP5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ACP5 expression by Western blot in cell or tissue lysates, Detect ACP5 in FFPE tissue sections by immunohistochemistry, Localize ACP5 by immunofluorescence/immunocytochemistry in cultured cells, Enrich ACP5 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 37 kDa; calculated MW: 58206 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 37 kDa
- Cellular localization (provided): Endoplasmic reticulum lumen .
- Tissue details (provided): Highly expressed in pancreas (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
