| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tartrate-resistant acid phosphatase type 5; TR-AP; Purple acid phosphatase; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase; ACP5; TRAP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEGH-1 is a rabbit monoclonal antibody that recognizes tartrate-resistant acid phosphatase type 5 (gene ACP5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 36.6 kDa, and the supplier reports an observed band at about 37 kDa.
Tartrate-resistant acid phosphatase type 5 (ACP5, TRAP) is an iron-containing phosphatase highly expressed in osteoclasts and activated macrophages. It dephosphorylates bone matrix proteins such as osteopontin, and TRAP staining or serum TRAP5b is a standard marker of osteoclasts and bone resorption.
| Target | Tartrate-resistant acid phosphatase type 5 (gene ACP5; UniProt P13686, human) |
|---|---|
| Clone | AEGH-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.6 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human tartrate Resistant Acid Phosphatase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes tartrate-resistant acid phosphatase type 5 as secreted and also located in the lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
