| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock protein 75 kDa, mitochondrial;HSP 75;TNFR-associated protein 1;Tumor necrosis factor type 1 receptor-associated protein;TRAP-1;TRAP1;HSP75; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human TRAP1 recombinant protein (Position: A571-H704). Human TRAP1 shares 91.7% and 94% amino acid (aa) sequence identity with mouse and rat TRAP1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TRAP1 (Heat shock protein 75 kDa, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TRAP1 Antibody Picoband® catalog # PB9834. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human TRAP1 recombinant protein (Position: A571-H704). Human TRAP1 shares 91.7% and 94% amino acid (aa) sequence identity with mouse and rat TRAP1, respectively. (reported region: A571-H704).
- Molecular weight context: reported MW: 80 kDa; calculated MW: 80110 MW
- Reactivity: Human,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Heat shock protein 75 kDa, mitochondrial; Heat shock protein 75 kDa, mitochondrial. Heat shock protein 75 kDa, mitochondrial is a protein that in humans is encoded by the TRAP1 gene. It is mapped to 16p13.3. This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. And this protein may function in regulating cellular stress responses. In addition, it was found that TRAP1 interacted with the N-terminal half of TNFR1. Also, TRAP1 interacted with the C-terminal ends of the proteins encoded by both multiple exostoses-causing genes, EXT1 and EXT2, but not with EXTL1 or EXTL3. Functional note: Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. . Reported localization: Mitochondrion . Mitochondrion inner membrane . Mitochondrion matrix . Expression/tissue context: Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is higly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors. .
Research relevance and current trends
- Metabolism: Researchers commonly examine how TRAP1 (Heat shock protein 75 kDa, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Metabolism Processes: Researchers commonly examine how TRAP1 (Heat shock protein 75 kDa, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Mitochondrial: Researchers commonly examine how TRAP1 (Heat shock protein 75 kDa, mitochondrial) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TRAP1 (Heat shock protein 75 kDa, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of TRAP1 (Heat shock protein 75 kDa, mitochondrial) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
