| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trafficking protein particle complex subunit 2; Sedlin; TRAPPC2; SEDL |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRAPPC2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28T69 is a rabbit monoclonal antibody that recognizes trafficking protein particle complex subunit 2 (gene TRAPPC2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 16.4 kDa, and the supplier reports an observed band at about 16 kDa.
TRAPPC2 (also called sedlin) is a subunit of the TRAPP complexes that tether vesicles and activate Rab GTPases along the secretory pathway. It is involved in transport from the endoplasmic reticulum to the Golgi, including export of large cargo such as procollagen. TRAPPC2 mutations cause X-linked spondyloepiphyseal dysplasia tarda.
| Target | Trafficking protein particle complex subunit 2 (gene TRAPPC2; UniProt P0DI81, human) |
|---|---|
| Clone | 28T69 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.4 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TRAPPC2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places trafficking protein particle complex subunit 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, fetal cartilage, fibroblasts, placenta and lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
