| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trefoil factor 3; Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B; TFF3; ITF; TFI |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Trefoil Factor 3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20T95 is a rabbit monoclonal antibody that recognizes trefoil factor 3 (gene TFF3) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 10.2 kDa, and the supplier reports an observed band at about 10 kDa.
Trefoil factor 3 (TFF3, also called intestinal trefoil factor) is a small secreted peptide made mainly by goblet cells of the intestine. It supports maintenance and repair of the intestinal lining by promoting epithelial cell migration over wounds, and it is studied in gut barrier function and cancer.
| Target | Trefoil factor 3 (gene TFF3; UniProt Q07654, human) |
|---|---|
| Clone | 20T95 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.2 kDa |
| Observed MW | 10 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human colon cancer.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human trefoil Factor 3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places trefoil factor 3 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in goblet cells of the intestines and colon (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
