| Field | Specification |
|---|---|
| Target | |
| Alternative names | TIR domain-containing adapter molecule 1;TICAM-1;Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TIR domain-containing adapter protein inducing IFN-beta;Ticam1;Trif; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse TRIF, different from the related human sequence by twelve amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets TIR domain-containing adapter molecule 1 (gene Ticam1) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 79.2 kDa, and the supplier reports an observed band at about 110 kDa.
TRIF (TICAM1) is a TIR-domain adaptor protein used by Toll-like receptors TLR3 and TLR4 in a MyD88-independent pathway. It recruits TBK1 and other kinases to activate IRF3 and NF-κB, leading to type I interferon and inflammatory cytokine production, and can also trigger cell death. TRIF is studied in antiviral immunity, endotoxin responses and innate immune signaling.
| Target | TIR domain-containing adapter molecule 1 (gene Ticam1; UniProt Q80UF7, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 79.2 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat testis tissue, mouse testis tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of mouse TRIF. It differs from the human sequence at twelve residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places TIR domain-containing adapter molecule 1 in the cytoplasm, cytoplasmic vesicle and mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yingwei Wu et al. (2019) TLR4 mediates upregulation and sensitization of TRPV1 in primary afferent neurons in 2,4,6-trinitrobenzene sulfate-induced colitis. Molecular Pain. 10.1177/1744806919830018
