| Field | Specification |
|---|---|
| Target | |
| Alternative names | TLR4 interactor with leucine rich repeats; Leucine-rich repeat-containing protein KIAA0644; TRIL; KIAA0644 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRIL recombinant protein (Position: V26-Q696). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets TLR4 interactor with leucine rich repeats (gene TRIL) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 88.7 kDa, and the supplier reports an observed band at about 89 kDa.
TRIL (TLR4 interactor with leucine-rich repeats) is a transmembrane protein that associates with the TLR4 receptor complex. It helps mediate innate immune responses to bacterial lipopolysaccharide, contributing to cytokine release, and is expressed notably in the brain, where it is studied in neuroinflammation.
| Target | TLR4 interactor with leucine rich repeats (gene TRIL; UniProt Q7L0X0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 88.7 kDa |
| Observed MW | 89 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TRIL fragment (Val26–Gln696), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TLR4 interactor with leucine rich repeats in the membrane. Tissue expression noted by UniProt (human): Highly expressed in the brain, ovary, small intestine and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yeke Wu et al. (2024) A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117738
Li Bo et al. (2026) Cyclopeptide heterophyllin from Pseudostellaria heterophylla alleviates neuroinflammation: a study integrating molecular dynamics, inflammatory pathway analysis, and in vivo validation. Food Science and Biotechnology. 10.1007/s10068-026-02223-8
