| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase TRIM16; Estrogen-responsive B box protein; Tripartite motif-containing protein 16; Trim16; Ebbp |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse TRIM16 recombinant protein (Position: D65-K210). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets E3 ubiquitin-protein ligase TRIM16 in mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 62.9 kDa, and the supplier reports an observed band at about 63 kDa.
TRIM16 is a tripartite motif E3 ubiquitin ligase that coordinates the cell's response to damaged lysosomes and phagosomes. It helps recruit autophagy machinery to damaged membranes and regulates the p62–KEAP1–NRF2 pathway to control oxidative stress and aggresome turnover, so it is studied in autophagy, xenophagy and stress responses.
| Target | E3 ubiquitin-protein ligase TRIM16 (gene Trim16; UniProt Q99PP9, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 62.9 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat testis tissue, rat C6 cells, mouse NIH/3T3 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HEPA1–6 cells.
Immunogen
Recombinant mouse TRIM16 fragment (Asp65–Lys210), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places E3 ubiquitin-protein ligase TRIM16 in the cytoplasm. Tissue expression noted by UniProt (mouse): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yingze Li et al. (2025) Intracellular Mechanical Stress-Mediated Autophagy Cell Death via Nanospikes for Cancer Treatment. Advanced Science. 10.1002/advs.202512256
Rong Zheng et al. (2023) Cordyceps cicadae polysaccharides attenuate diabetic nephropathy via the miR-30a-3p/TRIM16 axis. Journal of Diabetes Investigation. 10.1111/jdi.14116
