Anti-TRIM24 Antibody Picoband®

Overview

Anti-TRIM24 Antibody Picoband® is an antibody for TRIM24 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: TRIM24 (thrombospondin 2); UniProt: O15164
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 130 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-TRIM24 Antibody Picoband® catalog # A03258-3.

Biological background

Biological context: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.

Expression and localization notes: cellular localization: Synapse. Postsynaptic density. Dendritic spine. Dendrite., tissue context: High expression in invertebral disk tissue..

Common research applications

• Western blotting (WB): Compare TRIM24 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of TRIM24 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify TRIM24-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Tripartite motif-containing 24 (TRIM24) also known as transcriptional intermediary factor 1α (TIF1α) is a protein that, in humans, is encoded by the TRIM24 gene. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Synapse. Postsynaptic density. Dendritic spine. Dendrite.
• Tissue details: High expression in invertebral disk tissue.
• Research category: Calcium Signaling,Calmodulin Pathway,Cardiovascular,Hypertrophy,Neuroscience,Neurotransmission,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.