| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tripartite motif-containing protein 29; Ataxia telangiectasia group D-associated protein; TRIM29; ATDC |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRIM29 recombinant protein (Position: E169-R549). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tripartite motif-containing protein 29 (gene TRIM29) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 65.8 kDa, and the supplier reports an observed band at about 70 kDa.
TRIM29 (ATDC) is a tripartite motif protein expressed in epithelial and immune cells. In alveolar macrophages and other cells, it limits innate immune responses by promoting degradation of the NF-κB regulator NEMO and of STING, dampening interferon and inflammatory cytokine production. TRIM29 is studied in respiratory infection and squamous carcinomas.
| Target | Tripartite motif-containing protein 29 (gene TRIM29; UniProt Q14134, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 65.8 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells, human Hacat cells, human SIHA cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
Recombinant human TRIM29 fragment (Glu169–Arg549), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tripartite motif-containing protein 29 in the cytoplasm and lysosome. Tissue expression noted by UniProt (human): Expressed in placenta, prostate and thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
