| Field | Specification |
|---|---|
| Target | |
| Alternative names | BDNF/NT-3 growth factors receptor; GP145-TrkB/GP95-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Ntrk2; Trkb |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TrkB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22N08 is a rabbit monoclonal antibody that recognizes BDNF/NT-3 growth factors receptor (gene Ntrk2) in mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 92.1 kDa, and the supplier reports an observed band at about 120–140 kDa.
TrkB (NTRK2) is a receptor tyrosine kinase activated mainly by BDNF and neurotrophin-4. Its signaling supports neuron survival, differentiation and synapse formation and is central to activity-dependent synaptic plasticity, so TrkB is widely studied in learning and memory, depression, neurodegeneration and obesity.
| Target | BDNF/NT-3 growth factors receptor (gene Ntrk2; UniProt P15209, mouse) |
|---|---|
| Clone | 22N08 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.1 kDa |
| Observed MW | 120–140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human trkB.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places BDNF/NT-3 growth factors receptor in the cell membrane, endosome membrane, early endosome membrane and cell projection. Tissue expression noted by UniProt (mouse): Expressed in the brain, in neurons (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yajun Qiao et al. (2025) Identification and Validation of Antidepressant Small Molecules Using Bioinformatics and Mouse Depression Models. Drug Design Development and Therapy. 10.2147/DDDT.S537918
Wen Yuxin et al. (2024) Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance. Molecular Neurobiology. 10.1007/s12035-023-03860-4
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Hui-Yuan Yi et al. (2025) Brain-derived neurotrophic factor in the ventrolateral orbital cortex regulates spared nerve injury-induced neuropathic pain and related emotional disorders in mice. Neuroscience. 10.1016/j.neuroscience.2025.08.032
Shan Gao et al. (2017) Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain-derived neurotrophic factor. Cell Biochemistry and Function. 10.1002/cbf.3283
C. Li et al. (2011) The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm. Theriogenology. 10.1016/j.theriogenology.2011.09.003
