| Field | Specification |
|---|---|
| Target | |
| Alternative names | NT-3 growth factor receptor;2.7.10.1;GP145-TrkC;Trk-C;Neurotrophic tyrosine kinase receptor type 3;TrkC tyrosine kinase;NTRK3;TRKC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TrkC, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NT-3 growth factor receptor (gene NTRK3) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 94.4 kDa, and the supplier reports an observed band at about 85–91 kDa.
TrkC (NTRK3) is the receptor tyrosine kinase for neurotrophin-3. Ligand binding triggers autophosphorylation and activation of PI3K/AKT and MAPK signaling that supports cell survival and differentiation, and TrkC is studied in nervous system development, proprioceptive neurons and NTRK fusion-positive tumors.
| Target | NT-3 growth factor receptor (gene NTRK3; UniProt Q16288, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 94.4 kDa |
| Observed MW | 85–91 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat, Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, U87 cells, SHG cells, NEURO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human mammary cancer tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human trkC. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NT-3 growth factor receptor in the membrane. Tissue expression noted by UniProt (human): Widely expressed but mainly in nervous tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
