| Field | Specification |
|---|---|
| Target | |
| Alternative names | tRNA; adenine(58)-N(1); -methyltransferase non-catalytic subunit TRM6; mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6; tRNA(m1A58)-methyltransferase subunit TRM6; tRNA(m1A58)MTase subunit TRM6; TRMT6; KIAA1153; TRM6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRMT6 recombinant protein (Position: E6-K489). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tRNA (gene TRMT6) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 55.8 kDa, and the supplier reports an observed band at about 60 kDa.
TRMT6 is the non-catalytic, RNA-binding partner of TRMT61A in the enzyme that installs N1-methyladenosine (m1A) at position 58 of tRNAs, including initiator methionyl-tRNA. The same complex modifies a small set of mRNAs at tRNA-like structures, and TRMT6 is studied in tRNA stability, translation initiation and cell growth.
| Target | TRNA (gene TRMT6; UniProt Q9UJA5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 55.8 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human placenta tissue, human Daudi cells, human A431 cells, human U251 cells, human SH-SY5Y cells, human MOLT-4 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cnacer tissue, human cervical cancer tissue, human colon cancer tissue, human liver cancer tissue, human testis cancer tissue, human ovarian cancer tissue, hunan lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with confirmed staining (ICC/IF): human ovarian cancer tissue, human breast cancer tissue, human esophageal squamous cells carcinoma tissue.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human TRMT6 fragment (Glu6–Lys489), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tRNA in the nucleus. Tissue expression noted by UniProt (human): Expressed in brain, liver, testis and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
