| Field | Specification |
|---|---|
| Target | |
| Alternative names | tRNA; adenine(58)-N(1); -methyltransferase catalytic subunit TRMT61A; mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A; tRNA(m1A58)-methyltransferase subunit TRMT61A; tRNA(m1A58)MTase subunit TRMT61A; TRMT61A; C14orf172; TRM61 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRMT61A recombinant protein (Position: M1-G289). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tRNA (gene TRMT61A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 31.4 kDa, and the supplier reports an observed band at about 31 kDa.
TRMT61A is the catalytic subunit of the TRMT6–TRMT61A methyltransferase, which forms N1-methyladenosine (m1A) at position 58 in the T-loop of tRNAs, including initiator methionyl-tRNA. The complex also methylates a limited set of mRNAs, making TRMT61A a subject of research on tRNA modification and the m1A epitranscriptome.
| Target | TRNA (gene TRMT61A; UniProt Q96FX7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 31.4 kDa |
| Observed MW | 31 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PC-3 cells, human Caco-2 cells, human MCF-7 cells, human Hacat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human colon adenocarcinoma tissue, human larynx squamous cells carcinoma tissue, human liver cancer tissue, human lung adenocarcinom tissue, human ovarian serous cancer tissue, human prostate adenocarcinoma tissue, human renal cancer tissue, human spleen tissue, human thyroid cancer tissue, mouse brain tissue, mouse kidney tissue, mouse spleen tissue, rat colon tissue, rat kidney tissue, rat skin tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human ovarian cancer tissue.
Samples with a confirmed signal (flow cytometry): PC-3 cells, HepG2 cells.
Immunogen
Recombinant human TRMT61A fragment (Met1–Gly289), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tRNA in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
