| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Set1/Ash2 histone methyltransferase complex subunit ASH2;ASH2-like protein;ASH2L;ASH2L1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human TROY |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TNFRSF19 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25T43; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-TROY Rabbit Monoclonal Antibody catalog # M06157-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TNFRSF19 (Set1/Ash2 histone methyltransferase complex subunit ASH2).
- Antibody format: Monoclonal; clone 25T43; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TNFRSF19 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. . Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer Susceptibility,Chromatin Modifying Enzymes,Chromatin Remodeling,Epigenetics and Nuclear Signaling,Methylation,Proto-Oncogenes,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate TNFRSF19 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TNFRSF19 expression by Western blot in cell or tissue lysates, Detect TNFRSF19 in FFPE tissue sections by immunohistochemistry, Quantify TNFRSF19-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 46 kDa; calculated MW: 68723 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 46 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
