| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5,6-dihydroxyindole-2-carboxylic acid oxidase; DHICA oxidase; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1; TYRP1; CAS2; TYRP; TYRRP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRP1 Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEEB-20 is a rabbit monoclonal antibody that recognizes 5,6-dihydroxyindole-2-carboxylic acid oxidase (gene TYRP1) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 60.7 kDa, and the supplier reports an observed band at about 75 kDa.
Tyrosinase-related protein 1 (TYRP1) is a melanosomal enzyme of the tyrosinase family that oxidizes DHICA in the eumelanin pathway. It helps determine the type and amount of melanin produced and stabilizes tyrosinase, is used as a melanocyte marker, and its mutation causes oculocutaneous albinism type 3.
| Target | 5,6-dihydroxyindole-2-carboxylic acid oxidase (gene TYRP1; UniProt P17643, human) |
|---|---|
| Clone | AEEB-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 60.7 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human melanoma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TRP1 Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 5,6-dihydroxyindole-2-carboxylic acid oxidase in the melanosome membrane. Tissue expression noted by UniProt (human): Pigment cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
