| Field | Specification |
|---|---|
| Target | |
| Alternative names | Short transient receptor potential channel 1; TrpC1; Transient receptor protein 1; TRP-1; TRP1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRPC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21T11 is a rabbit monoclonal antibody that recognizes short transient receptor potential channel 1 (gene TRPC1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 91.2 kDa, and the supplier reports an observed band at about 120 kDa.
TRPC1 is a member of the canonical transient receptor potential (TRPC) family of cation channels that let calcium and sodium enter cells after receptor stimulation. It often assembles with TRPC4 or TRPC5 into heteromeric channels with altered properties, and it is studied in store-operated and receptor-operated calcium entry in many cell types.
| Target | Short transient receptor potential channel 1 (gene TRPC1; UniProt P48995, human) |
|---|---|
| Clone | 21T11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 91.2 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human PC-3 cells, human HepG2 cells, human Caco-2 cells, human U251 cells, rat testis tissue, mouse lung tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TRPC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places short transient receptor potential channel 1 in the cell membrane. Tissue expression noted by UniProt (human): Seems to be ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
