| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Short transient receptor potential channel 5; TrpC5; Transient receptor protein 5; TRP-5; hTRP-5; hTRP5; TRPC5; TRP5 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human TRPC5 recombinant protein (Position: L378-L973). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TRPC5 Antibody Picoband® is an antibody for TRPC5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TRPC5 (transient receptor potential cation channel subfamily C member 5); UniProt: Q9UL62
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 111 kDa, calculated 91791 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-TRPC5 Antibody Picoband® catalog # A02701.
Biological background
Biological context: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion.
Expression and localization notes: cellular localization: Cell membrane. Multi-pass membrane protein., tissue context: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole..
Common research applications
- Western blotting (WB): Compare TRPC5 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TRPC5 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify TRPC5-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Short transient receptor potential channel 5 (TrpC5) also known as transient receptor protein 5 (TRP-5) is a protein that in humans is encoded by the TRPC5 gene. It is mapped to Xq23. This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na (+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Multi-pass membrane protein.
- Tissue details: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.
- Research category: Calcium Channels,Calcium Signaling,Metabolism,Neuroscience,Neurotransmission,Plasma Membrane,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
