| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily M member 5;Long transient receptor potential channel 5;LTrpC-5;LTrpC5;MLSN1- and TRP-related gene 1 protein;TRPM5 ;LTRPC5 , MTR1 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRPM5 recombinant protein (Position: S1021-Q1108). Human TRPM5 shares 87.5% amino acid (aa) sequence identity with mouse TRPM5. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transient receptor potential cation channel subfamily M member 5 (gene TRPM5) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 131.5 kDa, and the supplier reports an observed band at about 131 kDa.
TRPM5 is a calcium-activated monovalent cation channel best known from taste receptor cells. Downstream of sweet, bitter and umami receptors, released calcium opens TRPM5, and the resulting sodium influx depolarizes the cell to transmit the taste signal. It is also expressed in pancreatic β cells and chemosensory cells, where it is studied in insulin release and sensing.
| Target | Transient receptor potential cation channel subfamily M member 5 (gene TRPM5; UniProt Q9NZQ8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 131.5 kDa |
| Observed MW | 131 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): SMMC cells, 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat testis tissue, human prostatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human TRPM5 fragment (Ser1021–Gln1108), expressed in E. coli. Sequence identity with the mouse ortholog: 87.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transient receptor potential cation channel subfamily M member 5 in the cell membrane. Tissue expression noted by UniProt (human): Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tianwen Yao et al. (2024) Exploring the therapeutic mechanism of Yuebi decoction on nephrotic syndrome based on network pharmacology and experimental study. Aging-US. 10.18632/aging.206116
