| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily M member 7; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; TRPM7; CHAK1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRPM7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADGD-20 is a rabbit monoclonal antibody that recognizes transient receptor potential cation channel subfamily M member 7 (gene TRPM7) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 212.7 kDa, and the supplier reports an observed band at about 213 kDa.
TRPM7 is an unusual protein that combines a divalent cation channel with its own C-terminal α-kinase domain. The channel conducts magnesium, calcium and zinc and is important for cellular magnesium and zinc balance, while the kinase can phosphorylate proteins such as annexin A1 and myosin II. It is studied in development, immune function, cell migration and proliferation.
| Target | Transient receptor potential cation channel subfamily M member 7 (gene TRPM7; UniProt Q96QT4, human) |
|---|---|
| Clone | ADGD-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 212.7 kDa |
| Observed MW | 213 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TRPM7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transient receptor potential cation channel subfamily M member 7 in the cell membrane and cytoplasmic vesicle membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jia Tian et al. (2021) Sacubitril Ameliorates Cardiac Fibrosis Through Inhibiting TRPM7 Channel. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2021.760035
Xueting Fan et al. (2023) miR-135a Regulates Atrial Fibrillation by Targeting Smad3. Cardiovascular Therapeutics. 10.1155/2023/8811996
