| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily M member 8;Long transient receptor potential channel 6;LTrpC-6;LTrpC6;Transient receptor potential p8;Trp-p8;TRPM8;LTRPC6, TRPP8; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TRPM8, different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
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| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody targets transient receptor potential cation channel subfamily M member 8 (gene TRPM8) in human samples and is validated for Western blot. The predicted molecular weight is 127.7 kDa, and the supplier reports an observed band at about 127 kDa.
TRPM8 is the main cold- and menthol-activated ion channel of sensory neurons. It opens at cool temperatures and in response to cooling compounds such as menthol and icilin, allowing cation entry that signals cold, and it is also involved in regulating tear secretion. It is studied in thermosensation, cold pain and prostate biology.
| Target | Transient receptor potential cation channel subfamily M member 8 (gene TRPM8; UniProt Q7Z2W7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 127.7 kDa |
| Observed MW | 127 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells, 22RV1 cells, SW620 cells, A549 cells, A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 40 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human TRPM8. It differs from the mouse sequence at four residues and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transient receptor potential cation channel subfamily M member 8 in the cell membrane, membrane raft and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed in prostate. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Zhang et al. (2024) Neuregulin 1 mitigated prolactin deficiency through enhancing TRPM8 signaling under the influence of melatonin in senescent pituitary lactotrophs. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.133659
Margherita Luongo et al. (2020) Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic Ductal Adenocarcinoma Cell Lines. Cancers. 10.3390/cancers12102774
Zhang Wei et al. (2022) Comprehensive Pan-Cancer Analysis of TRPM8 in Tumor Metabolism and Immune Escape. Frontiers in Oncology. 10.3389/fonc.2022.914060
Zhi Wang et al. (2023) Differential expressions of hypothalamic thermosensitive TRP ion channels may underlie the posthatching ontogeny of brain cooling capacity in broiler chickens. Poultry Science. 10.1016/j.psj.2023.102782
