| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily V member 1; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; VR1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TRPV1 recombinant protein (Position: E107-A800). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets transient receptor potential cation channel subfamily V member 1 (gene TRPV1) in human and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 95.0 kDa, and the supplier reports an observed band at about 95 kDa.
TRPV1, the capsaicin receptor, is a calcium-permeable cation channel on pain-sensing neurons that is activated by noxious heat, acidic pH and vanilloids such as capsaicin. Inflammatory signals sensitize the channel, so it plays a key role in inflammatory pain and heat hyperalgesia, and it can also respond to endocannabinoids such as anandamide.
| Target | Transient receptor potential cation channel subfamily V member 1 (gene TRPV1; UniProt Q8NER1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.0 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HepG2 cells, rat liver tissue, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): T-47D cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human TRPV1 fragment (Glu107–Ala800), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places transient receptor potential cation channel subfamily V member 1 in the postsynaptic cell membrane and cell membrane. Tissue expression noted by UniProt (human): Widely expressed at low levels. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Junguang Wu et al. (2025) A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson’s disease. Science Advances. 10.1126/sciadv.ado4927
Chan Lu et al. (2023) Interaction of high temperature and NO2 exposure on asthma risk: In vivo experimental evidence of inflammation and oxidative stress. Science of the Total Environment. 10.1016/j.scitotenv.2023.161760
Beibei Lu et al. (2025) Preparation of dual supramolecular EGCG-carboxymethyl chitosan: Structure, antioxidant and anti-inflammatory properties. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.139523
Shuangling Wang et al. (2022) Ion channel-targeting near-infrared photothermal switch with synergistic effect for specific cancer therapy. Journal of Materials Chemistry B. 10.1039/D1TB02351A
Xiaochun Huang et al. (2025) TRPV1 mediates oxidative stress and autophagy pathways to regulate airway MUC5AC secretion. Biochemical Pharmacology. 10.1016/j.bcp.2025.117658
ZhiPeng Jiang et al. (2025) Targeting TRPV1 channels in desensitized neural afferent pathways may help mitigate pain and lower urinary tract symptoms caused by prostatitis. Frontiers in Pharmacology. 10.3389/fphar.2025.1541684
