| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily V member 3; TrpV3; Vanilloid receptor-like 3; VRL-3; TRPV3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TRPV3, which shares 77.8% amino acid (aa) sequence identity with mouse TRPV3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes transient receptor potential cation channel subfamily V member 3 (gene TRPV3) in human samples and is validated for Western blot. The predicted molecular weight is 90.6 kDa, and the supplier reports an observed band at about 100 kDa.
TRPV3 is a warmth-activated, calcium-permeable cation channel that is highly expressed in skin keratinocytes. Its response grows with rising temperature, and signaling through TRPV3 restrains keratinocyte proliferation in hair follicles and promotes hair follicle regression, so it is studied in skin barrier function, itch and hair growth.
| Target | Transient receptor potential cation channel subfamily V member 3 (gene TRPV3; UniProt Q8NET8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 90.6 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human MDA-MB-453 cells, human PC-3 cells, human Caco-2 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human TRPV3. Sequence identity with the mouse ortholog: 77.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transient receptor potential cation channel subfamily V member 3 in the cell membrane, cytoplasm and lysosome. Tissue expression noted by UniProt (human): Abundantly expressed in CNS. Widely expressed at low levels. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
