| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC; ECaC1; Osm-9-like TRP channel 3; OTRPC3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRPV5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27T91 is a rabbit monoclonal antibody that recognizes transient receptor potential cation channel subfamily V member 5 (gene TRPV5) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 82.6 kDa, and the supplier reports an observed band at about 100 kDa.
TRPV5 is a highly calcium-selective channel of the vanilloid TRP subfamily found mainly in the distal kidney tubule. It is constitutively open at low intracellular calcium and provides the entry step for active calcium reabsorption from urine, with calcium-dependent feedback limiting its activity. It can combine with TRPV6 and is studied in calcium homeostasis.
| Target | Transient receptor potential cation channel subfamily V member 5 (gene TRPV5; UniProt Q9NQA5, human) |
|---|---|
| Clone | 27T91 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 82.6 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human RT4 cells, human THP-1 cells, human Jurkat cells, rat thymus tissue, rat kidney tissue, mouse thymus tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human TRPV5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transient receptor potential cation channel subfamily V member 5 in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guo Ge et al. (2025) PHPT1 acts as an inhibitor in high-altitude pulmonary hypertension via negative TRPV5 signaling regulation. Journal of Translational Medicine. 10.1186/s12967-025-06980-8
Jin Zhang et al. (2023) Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5. Journal of Dairy Science. 10.3168/jds.2022-23218
