| Field | Specification |
|---|---|
| Target | |
| Alternative names | Elongation factor Ts, mitochondrial; EF-Ts; EF-TsMt; TSFM |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TSFM |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29T44 is a rabbit monoclonal antibody that recognizes elongation factor Ts, mitochondrial (gene TSFM) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 35.4 kDa, and the supplier reports an observed band at about 32 kDa.
TSFM is mitochondrial elongation factor Ts, a nucleotide exchange factor for mitochondrial EF-Tu (TUFM). By swapping GDP for GTP, it recycles EF-Tu so that it can deliver new aminoacyl-tRNAs to the ribosome, and it is therefore required for mitochondrial protein synthesis; variants cause combined oxidative phosphorylation deficiency.
| Target | Elongation factor Ts, mitochondrial (gene TSFM; UniProt P43897, human) |
|---|---|
| Clone | 29T44 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.4 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TSFM.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places elongation factor Ts, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
